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On-line Analytical Workflow for Automated Monitoring of mAb Glycosylation during Biomanufacturing
Schematic of N-GLYcanyzer PAT Workflow for Integrated Upstream Analysis of Monoclonal Antibody Titer and N-linked Glycosylation
Invention Summary:
Continuous process for biologic manufacturing in biopharmaceutical industry relies on prompt feedback of process parameters for decision making as they may quickly influence the critical quality...
Published: 9/1/2023
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Inventor(s): Shishir Chundawat, Aron Gyorgypal
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering
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Engineered Hydrolases for Biofuel Production from Cellulosic Biomass
Cellulases with mutations to supercharge the CBM (D3, D4) degrade 2.5-fold more cellulose after 48 hours at elevated temperatures
Invention Summary:
Cellulosic biomass is a substantial fuel source with ~200 billion tons of plant biomass produced yearly. However, releasing sugars from biomass by enzymatic degradation using Carbohydrate Active...
Published: 8/8/2023
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Inventor(s): Shishir Chundawat, Bhargava Nemmaru, Antonio De Chellis, Deanne Sammond
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Energy & Battery
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Synthetic Promoter-Based Azide Biosensor & Protein Induction System
(A) Escherichia coli BW25113-Wt cells with pCyn-v2-GFP plasmid were induced with varying azide concentrations and GFP fluorescence of cell lysate was measured over 24 hours. (B) E. coli BL21 contaning control pEC-GFP plasmid was induced with IPTG (isopropyl β-d-1-thiogalactopyranoside). (C). BW25113-Wt cells with pCyn-v2-GFP plasmid was incubated...
Published: 10/12/2022
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Inventor(s): Shishir Pratap Singh Chundawat, Chandra Bandi, Kyle Skalenko
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Food, Nutrition and Nutraceuticals
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High-Throughput Screening of Glycosynthases
Invention Summary:
The ability to synthesize specific glycosidic linkages provides a way to produce designer glycans. Glycosytransferases that synthesize oligosaccharides are expressed poorly, have narrow substrate specificity and use expensive nucleotide sugars, limiting their utility in scaling-up glycan synthesis. Glycosynthases (GSs) offer an alternative...
Published: 10/12/2022
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Inventor(s): Shishir Pratap Singh Chundawat, Ayushi Agrawal, Chandra Bandi
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Food, Nutrition and Nutraceuticals
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Engineered GH for Improved Glycan Polymer Synthesis
Invention Summary:
Oligosaccharides and polysaccharides (or glycans) are complex macromolecules in living systems that serve critical structural and functional roles. In vitro synthesis of these complex biomolecules is very challenging. Glycosytransferases are naturally available enzymes that catalyze the synthesis of glycans, but have poor expression...
Published: 10/12/2022
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Inventor(s): Shishir Pratap Singh Chundawat, Chandra Bandi
Keywords(s):
Category(s): Technology Classifications > Physical Sciences & Engineering, Technology Classifications > Healthcare & Life Sciences, Technology Classifications > Food, Nutrition and Nutraceuticals
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